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While maintaining a scholar's attention to the complexities of the original, Hinton here recreates Tao Chien as a compelling poetic voice in English.more As David Hinton writes in his introduction, Tao Chien "stands at the head of the great Chinese tradition like a revered grandfather: profoundly wise, self-possessed, quiet, comforting." Tao was the first writer to make a poetry of his natural voice and immediate experience, thereby creating the personal lyricism which distinguishes ancient Chinese poetry and makes it seem so contemporary. THE SELECTED POEMS OF TAO CHIN brings into English some of the most important poetry in all of Chinese literature. As David Hinton writes in his introduction, Tao Chien "stands at the head of the great Chinese tradition like a revered grandfather: profoundly wise, self-possessed, quiet, comforting." Tao was the first writer to make a poetry of his natural voice and
Tao ming se how to#
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Tao ming se code#
Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction Our new method has broad applications for the site-specific labeling and smFRET measurement of membrane protein complexes, and the elucidation of their dynamic properties such as transducer protein selection. Using this strategy, we monitored the subtle but functionally important conformational change of βarr1 upon activation by the G-protein coupled receptor (GPCR) through smFRET for the first time. Here, we demonstrate the site-selective incorporation of a novel unnatural amino acid (2-amino-3-(4-hydroselenophenyl) propanoic acid, SeF) through genetic expansion followed by a Se-click reaction to conjugate the Bodipy593 fluorophore on calmodulin (CaM) and β-arrestin-1 (βarr1). However, the success of protein smFRET relies on the precise and efficient labeling of two or more fluorophores on the protein of interest (POI), which has remained highly challenging, particularly for large membrane protein complexes. Single-molecule Förster resonance energy transfer (smFRET) is a powerful tool for investigating the dynamic properties of biomacromolecules. School of Biological Science and Technology, University of Jinan, Jinan, Shandong, China School of Life Sciences, University of Science and Technology of China, Baohe District, Anhui, China Shenzhen Institute of Transfusion Medicine, Shenzhen Blood Center, Futian District, Shenzhen, China University of the Chinese Academy of Sciences (UCAS), Shijingshan District, Beijing, China Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, 44 Wenhua Xi Road, Jinan, Shandong, ChinaĮ-mail: of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Tsinghua University, Haidian District, Beijing, ChinaĮ-mail: of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Haidian District, Beijing, China Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin Airport Economic Area, Tianjin, China